Difference between C8 and C18 column in HPLC Introduction-What Is a C8 Column A C8 column, also known as an octyl silane column, contains silica particles bonded with 8-carbon alkyl chains. These shorter chains make the column less hydrophobic than C18. Carbon chain length: 8 carbons Hydrophobicity: Moderate Retention time: Shorter Faster analysis compared to … Read more
HPLC Method Development Steps-New things in 2026 12-Step HPLC Method Development Guide for Beginners and Experts What Is HPLC Method Development: Follow HPLC Method Development Steps HPLC method development is the process of selecting and optimizing chromatographic conditions to separate, identify, and quantify analytes in a sample. The goal is to achieve Good peak separation, … Read more
What is an HPLC Column: HPLC Column Types Introduction: HPLC Column Types An HPLC column is a stainless steel or peek tube packed with stationary phase particles. When a liquid mobile phase carries a sample through the column, compounds interact differently with the stationary phase, leading to separation. Every HPLC column is defined by: (Stationary … Read more
