Polar and nonpolar solvent Introduction: What Is a Solvent A solvent is a substance that dissolves another substance (called the solute) to form a solution. The solvent is usually present in a larger amount than the solute. For example: Water dissolving salt, Alcohol dissolving perfumes, Hexane dissolving oils The ability of a solvent to dissolve … Read more
Diluent selection during method development of HPLC Diluent selection during method development of HPLC Why Diluent Selection Matters in HPLC High-Performance Liquid Chromatography (HPLC) is one of the most powerful analytical techniques used in pharmaceutical, chemical, food, and environmental laboratories. While much attention is given to column selection, mobile phase composition, and detector settings, diluent … Read more
Difference between C8 and C18 column in HPLC Introduction-What Is a C8 Column A C8 column, also known as an octyl silane column, contains silica particles bonded with 8-carbon alkyl chains. These shorter chains make the column less hydrophobic than C18. Carbon chain length: 8 carbons Hydrophobicity: Moderate Retention time: Shorter Faster analysis compared to … Read more
HPLC Method Development Steps-New things in 2026 12-Step HPLC Method Development Guide for Beginners and Experts What Is HPLC Method Development: Follow HPLC Method Development Steps HPLC method development is the process of selecting and optimizing chromatographic conditions to separate, identify, and quantify analytes in a sample. The goal is to achieve Good peak separation, … Read more
What is an HPLC Column: HPLC Column Types Introduction: HPLC Column Types An HPLC column is a stainless steel or peek tube packed with stationary phase particles. When a liquid mobile phase carries a sample through the column, compounds interact differently with the stationary phase, leading to separation. Every HPLC column is defined by: (Stationary … Read more
